Malonaldehyde (MDA) content was measured by the HPLC method as described by Agarwall
and Chase. An aliquot of 20 µl was injected onto a Shimadzu LC-2010HT with an
InertSustain C-18 column (4.6mm×150 mm, 5µm particle size, GL Sciences Inc. Tokyo,
Japan). The standard curve was prepared using 1,1,3,3-tetraethoxypropane. Thiobarbituric
acid-reacting substances (TBARS) were expressed as nmol per gram of wet tissue.