Leaf litter and leaf
fragments from the mud surface were collected, rinsed in ambient
brackish water, and plated onto dilute V8 seawater agar (40
ml V8 juice, 3 g CaCO3
, 16 g Bacto agar and 960 ml seawater)
amended with 25 ppm pimaricin, 100 ppm ampicillin, 25 ppm
rifampicin, 25 ppm pentachloronitrobenzene (PARP). Mycelium
was observed growing from leaf material into agar after 1–3 d