Identification of AAB based only on morphological, biochemical, and physiological characteristics is not reliable and, therefore, is insufficient because of the poor reproducibility and discriminatory power of these phenotypic tests . For this reason, nucleic acid-based molecular methods are now used to characterize and identify isolates of AAB from wine and vinegar ecosystems .These have included Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR), Repetitive Extragenic Palindromic-PCR (REP-PCR) (González et al.,, (GTG) 5 -rep-PCR fingerprinting and RAPD-PCR A reliable taxonomic identification is obtained when these techniques are combined with the sequencing of 16S rDNA genes and internal transcribed spacer sequences(ITS) of the 16S–23S rDNA genes. Restriction fragment length polymorphism (RFLP) analysis of the ribosomal genes or their spacer regions has also been used for the identification of AAB present in food-related ecosystems