TEAC value was determined according to
Miller et al. (1996) and Re et al. (1998). ABTS•+ cation
was generated through the interaction of 19.2 mg of
ABTS (2’2-azino-bis(3-ethylbenzotriazoline-6-sulfonic
acid)), dissolved in 5 mL of HPLC-grade water and
88 μL of potassium persulfate (K2S2O8) (0.0378 g
mL-1). It was incubated in the dark at room temperature
for 16 h; then 1 mL of ABTS activated radical was
taken and 88 mL of ethanol was added. The radical was
adjusted at an absorbance of 0.7±0.02 at 734 nm. The
reaction was initiated adding 2970 μL of ABTS•+ and 30 μL of the extract or Trolox standard solution in
methanol and. absorbance was monitored at 734 nm at
1 and 6 min. The percentage of inhibition was
calculated and results were expressed as μmol of
ET/100 gFW