Butter, a dairy product containing a large amount of milk fat, is often the object of economic fraud involving replacement with cheaper animal fats or vegetable oils (mainly palm oil). Determination of adulterants in butter continues to be an analytical challenge. In order to obtain a rapid method, the aim of this study was to evaluate the applicability of the DSC technique for quantitative assessment of butter adulteration with palm oil (PO). Validation of the developed DSC method required the determination of linearity and repeatability, as well as the accuracy by performing the recovery experiments. The addition of PO to butterfat (BF) results in an altered shape of the melting curve. With an increase of PO content in BF from 0% to 35%, the melting temperatures