ABSTRACT
Enterocin CCM4213 produced by bacteriocinogenic and probiotic Enterococcus faecium CCM4231
strain and sage plant extract were applied to rabbits separately and in the combination. A total of 96
rabbits (5-weeks old, male sex, HyPlus breed) were divided into three experimental (EG1, EG2, EG3)
groups and one control group (CG). Animals of EG1 received Salvia officinalis plant extract (10
µl/animal/day into water), rabbits of EG2 received partially purified bacteriocin (PPB)–enterocin
CCM4231 (50 µl/animal/day into water) produced by E. faecium CCM4231 strain, animals of EG3
received the combination of PPB CCM4231 (50 µl/animal/day into water) with sage plant extract (10
µl/animal/day into water) for 21 days. The experiment lasted 42 days. The animals were fed by the
complete granulated mixture. Faeces were sampled at the start of the experiment, at day 7 (1 week of
administration), at day 21 (3 weeks of administration) and 28 (1 week after cessation of application)
and at day 42 (3 weeks after cessation of application) to count rabbit intestinal microflora as well as
the occurence of Eimeria sp. oocysts. Samples of caecal contents (3 slaughtered animals of each
group) were collected at days 21 and 42 to determine caecal bacterial counts. To check phagocytic
activity, the animals were sampled at the start of the experiment, at days 21 and 42. The reduction of
E.coli in EG2 (difference 1.3 log10 CFU/g) was noted at day 7 as well as the reduction of Clostridiumlike
sp. (difference 1.57 log10 CFU/g) at day 42 in comparison to CG. Reductive effect against
Pseudomonas-like sp. (P<0.001) was also noted in EG3 at day 42 in comparison to CG. The other
bacteria were not influenced by additives. The bacterial counts in the caecum were lower than in the
faeces and no significant changes were noted. In the experimental groups prolonged
immunostimulative effect (P<0.001) was observed in comparison to CG at day 42. The most
pronounced anticoccidial effect was recorded in EG3 at day 7 compared to CG (in CG 65 OPG, in
EG3 10 OPG). The oocysts shedding were also reduced in EG2 (20 OPG) compared to CG (65 OPG).
At day 21, oocysts were also reduced in EG1 (30 OPG to CG - 1184OPG); in EG2 and EG3 were
found only rarely. Results of our experiment suggest possible commercial utilization of natural
additives tested.
Key words: Bacteriocin, Enterocin, Rabbit, Sage.
I
การแปล กรุณารอสักครู่..