Difference in dietary protein contents affects circadian rhythm of nutrient metabolism. In a recent report, it has been reported that a high protein meal given in the evening (40% of energy as protein) significantly increases the plasma free amino acids concentration measured on the next morning, thus even more than 12 hours after the meal (Nishioka et al., 2013). Compared with the normal group, a high protein meal fed in the morning and a low protein meal fed in the evening significantly increases the average daily gain (ADG) of growing pigs (Xie CY et al., 2014). In the past, ration formulation according to the dietary requirements of specific developmental phases has formed the basis of pig feeding practices. In the present study, we focused on the effects of a two-meal daily feeding pattern with varied levels of dietary protein on several parameters in pigs, including growth performance, blood biochemistry, and antioxidant indexes.