. Fluorescent filters
were set to pass light with an excitation wavelength of 485 nm and an
emission wavelength of 528 nm. In a final assay, 150 μL 0.08 μM sodium
fluorescein (fluorescent probe) was used as a target of free radical
damage, 25 μL 2,2′-azobis(2-amidino-propane) dihydrochloride
(AAPH) (4 × 10−3 M) as a peroxyl radical generator, 25 μL diluted
supernatants as sample, and Trolox as a control standard.