Under all conditions, 0.4 g/g (YX/S) was obtained, but when the YESP concentration was increased from 8.34 to 30.6 g/L, the pDNA volumetric yield and productivity increased from 210 to 273 mg pDNA/L and 17.2–22 mg pDNA/(L h), respectively, and the specific pDNA yield of 9.7 mg pDNA/g DCW was constant.