Analysis of CD products
CD-TCE complex precipitate was dissolved
with distilled water and boiled at 80
o
C for 10 min.
The solution was filtered through a 0.45 mm filter.
The 15 mL filtrate was applied onto Whatman #1
paper (22 x 22 cm). The paper sheet was separately
irrigated with 4:3:3 (v/v) of n-butanol-ethanol-water. The CDs on the paper sheet were located
using 1% methanolic iodine (Szejtli, 1988).