After the 21-day exposure period, 100 treated and control females were transferred to 2 L cubical aquaria containing treatment water and then visually presented with one of 14 live, untreated males in an adjacent aquarium. Previous studies have shown that transportation to a novel tank has no measurable effect on stress response in domesticated B. splendens [43]. The female fish were videotaped for 10 minutes and these recordings were scored for duration of three behavioral responses: latency to respond to the presence of the male, duration of opercular displays (tonic movement of the operculum and branchiostegal membrane), and duration of fin displays (erection of the dorsal and caudal fins). These displays are important parts of the agonistic and courtship repertoires of both female and male B. splendens [21, 25, 27]. Where , subjects were removed from the experiment because they died or showed signs of illness ( total, across all treatment groups).