2.2. Photobioreactor and cultivation conditions
Cells were cultured photoautotrophically with 3 L of f/2 medium in a 5-L circular cylindrical photobioreactor (radius, 8.2 cm; height, 27 cm) at 20 ± 1 C (first stage). Filtered air was supplied to the reactor through a 0.2-mm PTFE membrane at a rate of 2.5 L/min. Illumination for the 12/12-h light/dark cycle was provided at 108.9 mmol photon/m2/s light intensity using white fluorescent