Schematic representation of fish myocytes development and gene expression of myogenic factors. Proliferating cell nuclear antigen (PCNA) expression is higher in the proliferation phase (in blue) and is accompanied by the expression of different transcription factors (Sox8, Myf5, MyoD2 and Pax7). In the later stages, myogenin and MRF4 increased expression induces maturation of myocytes into myotubes. The expression of myosin heavy chain (MHC) confirms the differentiation process (in orange).