2.3. Determination of antifungal activity of extract against radial growth
The method developed by Astiti and Suprapta (2012) was used to measure the effect of extract of F. septica to
the radial growth of C. acutatum. Various concentrations of leaf extract of F. septica viz. 0%, 0%, 1%; 2%; 3%;
4% and 5 % (w/v) were put in Petri dishes and added with 10 ml melted PDA medium. The Petri dishes were
shaken gently to allow the extract to distribute evenly in the medium. A mycelial plug (5 mm diam.) of C.
acutatum taken from the edge of a 5-day old culture was put in the center of PDA.