For protoplast fusion it is important that the cell wall of plant and microorganisms is
degraded .So various enzymes used for this process.cellulase and pectinase or macerozyme
acting on plant cell wall.Bacterial cell wall are degraded by the action of lysozyme.Fungal
wall degraded by Novozyme -234 which includes glucanase and chitinase. Streptomyces cell
wall degraded by action of lysozyme and achromopeptidase