Prior to the experiment, F. benghalensis leaf was rinsed thoroughly
by deionized water. Leaf extract was prepared by taking 25 g of leaf
thoroughly washed, dried, cut into fine pieces, mixed with 100 mL
of deionized water in a 250 mL Erlenmeyer flask and mixture was
boiled for 5 min before decanting. For reduction of silver ions, 5 ml
of Ficus benghalensis leaf extract was added dropwise into 45 ml of
10−3 M aqueous solution of AgNO3 with constant stirring at 50–
60 C. As soon as, F. benghalensis extract was mixed in aqueous solution
of silver ion complex, it starts to change color from whitish to
yellowish brown due to excitation of surface plasmon resonance
which indicates the formation of silver nanoparticles. The synthesized
nanoparticles by F. benghalensis leaf extract were centrifuged at
5500 rpm for 15 min and subsequently redispersed in deionized
water to get rid of any uncoordinated biological molecules.