Media #1, 3 and 4 consisted mainly of salts with glucose, glycerol or both. Media #2, 5, 6 and 7 was composed of salts and different carbon sources: citric acid and glucose, glycerol or both. The results indicate that in all the experiments carried out using chemically defined media spore production was lower than in the experiment carried out using DSM. Within the experiments performed with chemically defined media the best results were obtained using media formulation #2, with higher carbon and nitrogen concentration. This media formulation was optimized by studying the influence of vitamin, nitrogen, carbon and calcium concentration on spore