These media allowed us to
simulate nutritional condition of early fermentation (high carbon and
nitrogen concentration), mid-fermentation (rate limiting nitrogen and
high concentration in carbon), and
‘
adjusted
’
fermentation for nitrogen
(rate limiting carbon and high concentration in nitrogen). A quantity of
100 mg/L of (+)-catechin hydrate (Fluka Analytical) was included to
make SCM and SLAD supplemented with a source of a polyphenolic
compound. Black grape skins were taken from the Calabrian grape cul-
tivars
Magliocco
and
Gaglioppo
and washed with water.