Investigation of the effect of the HS-EE on the
histological changes of pancreas
The normal and STZ-induced diabetic rats of
the above investigation, which received distilled water
or HS-EE 1.0 g/kg, were sacrificed by pentobarbitone
sodium injection (60mg/kg BW, i.p.). The pancreas was
dissected and fixed in 10% buffered formalin. The
organ was processed in graded series of alcohol and
embedded in paraffin wax. Serial sections of 5 μm
were cut using a microtome (American Optical, Model
82), mounted on glass slides and stained with
hematoxylin-eosin and photographed by Leitz
microscope. The number and size of islets of
Langerhans in pancreas were measured in 10 low power
fields (LPF) per section using Olympus DP25 and
Immage Proplus programe.