Experiment. 3: Intensity of UV irradiation.Fruit skin discs were irradiated at different
intensities of UV light. Skin discs measuring 2 cm in diameter and 3 mm in thickness were obtained from the equatorial part of each fruit. Three discs were placed in a 6 cm in diam sterile plastic petri dish (MS-11600; Sumi-tomo Bakelite Co., Ltd., Tokyo) containing 3 mL of a filter-sterilized solution of 0.4 M sucrose and 0.05% L-ascorbic acid on one layer of filter paper. The light source and the equipment were the same as those in Expt. 2.
The intensity of irradiation was adjusted by
placing several layers of shade screen over
the petri dish. The intensity was monitored
Materials and Methods
Plant materials. Yellow-green fruit were
collected at the beginning of stage III of fruit
Received for publication 12 Aug. 2002. Accepted for publication 10 Feb. 2003.
1Professor.
2Assistant professor.beneath the plastic cover. The cultures were continuously irradiated for 96 h.