At the peak, in 1985, my collection of microbial cultures had 2000 strains of 250 species. These are the only media I used for stock cultures. ** marks the media I use for many species, * marks media I use for a few species. I needed the other media only for rare species. Notice the abbreviation listed before each medium. I mark that abbreviation on each tube of the medium and use it on all my web pages. Notice I use spring water (well water or tap water; not distilled water). There is seldom any reason to purchase distilled water. I regret that most of these media use professional ingredients. The high school student can try to improvise see B041 for descriptions of tryptone, yeast extract, etc and ideas for substitutes. Besides being used for stock cultures, many of these media are excellent for classroom experiments. TGY is an excellent medium; most students and teachers can get by using it alone. If you have questions contact me at the e-address below. I autoclave all these media for 15 minutes at 15 pounds pressure.
AM * Acetobacter Medium