2.7. Statistical analysis
The method of 2−ΔΔCT was used to analyze the real-time PCR data expressed as the fold change relative to the average value of the control group (Livak and Schmittgen, 2001).
All data were expressed as mean±S.E.M.
All statistical analyses were performed with SPSS 13.0 for Windows.
The differences were tested with ANOVA using ttest for independent samples.
A P-valueb0.05 was considered significant.