In order to use themolecular fluorescence to monitor CF, a variety of
different fluorophores such as 8-hydroxyquinoline, dithizone,
chromazurol, rhodamine B and BSA were assayed. No variation on native
fluorescent response of mentioned fluorophores was observed on
studied experimental conditions in the presence of analyte CF, with
the exception of BSA that it showed quenching effect on fluorophore
emission. So, this fluorophore was selected to follow the optimization
studies.
Fig. 1. Schematic representation of FIA system designed for caffeine determination by the
developed methodology.
Fig.