The present work wss carried out to check the application of the test for several cereals and nut and to find out suitable working conditions for the DNA Comet Assay for identification of irradiated and non-irradiated samples. It should be emphasized,however,that DNA fragmentation can be caused by various chemical or physical treatments including ionizing radiation,and this means that the assay is not radiationspecific and can only used as a screening technique. But since it is a rapid and simple technique it is of great value to screen samples suspect of irrdaition by this rapid test,and consequently confirm positive results using a standardized method to specifically prove an irradiation treatment,e.g. using thermoluminescence measurements