The objective of this study is to investigate the effects of consecutive turnover of green manure
(ryegrass (Lolium perenne L.)) on soil microbial biomass C and N and enzyme activities. The results
showed that the incorporation of green manure in tobacco-planted fields generally significantly
enhanced enzyme activities (soil para nitrophenol (PNP), urease, catalase, and sucrase) and soil
microbial biomass contents. The extent of enhancement increased with the increase in consecutive
turnover. The C and N contents in microbial biomass were significantly lower in conventionally
cultivated soil than in soils from the green manure-incorporated field. This finding indicated that
incorporation of green manure could stimulate the activity of soil microorganisms. The average
improvement gained from the T3 treatment (three-year consecutive cultivation of green manure
since the harvest of tobacco) relative to the T0 treatment (conventional cultivation without any
green manure) over the whole growth period of tobacco was 1.34 to 1.52 times for urease activity,
1.11 to 1.19 times for PNP activity, 1.58 to 1.17 times for sucrase activity, and 1.24 to 1.50 times for
catalase activity. Overall, the activities of soil enzymes correlated well with the soil microbial biomass
C and N. This study provides guidance on the measurement of green manure to manage the activation
of soil microbial biomass and improve the quality of tobacco-planted soil.
The objective of this study is to investigate the effects of consecutive turnover of green manure(ryegrass (Lolium perenne L.)) on soil microbial biomass C and N and enzyme activities. The resultsshowed that the incorporation of green manure in tobacco-planted fields generally significantlyenhanced enzyme activities (soil para nitrophenol (PNP), urease, catalase, and sucrase) and soilmicrobial biomass contents. The extent of enhancement increased with the increase in consecutiveturnover. The C and N contents in microbial biomass were significantly lower in conventionallycultivated soil than in soils from the green manure-incorporated field. This finding indicated thatincorporation of green manure could stimulate the activity of soil microorganisms. The averageimprovement gained from the T3 treatment (three-year consecutive cultivation of green manuresince the harvest of tobacco) relative to the T0 treatment (conventional cultivation without anygreen manure) over the whole growth period of tobacco was 1.34 to 1.52 times for urease activity,1.11 to 1.19 times for PNP activity, 1.58 to 1.17 times for sucrase activity, and 1.24 to 1.50 times forcatalase activity. Overall, the activities of soil enzymes correlated well with the soil microbial biomassC and N. This study provides guidance on the measurement of green manure to manage the activationof soil microbial biomass and improve the quality of tobacco-planted soil.
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