Lipid oxidation studies were done in spaghetti enriched with LC n−3 PUFA by the addition to semolina of 1.2% ROPUFA 10 n−3 refined marine oil powder containing EPA and DHA. Two oxidative parameters were evaluated: peroxide value (PV), to assess primary oxidation and oxidised fatty acids to quantify secondary oxidation products. Functional spaghetti had a shelf life comparable to control pasta. LC n−3 PUFA were not significantly implicated in the onset of oxidation in spaghetti stored under daylight and accelerated oxidation in a laboratory heater. Storage decidedly affected shelf life: PV in functional spaghetti increased from 7.1 to 43.4 meq O2/kg of fat under light exposure over 12 months, and from 7.1 to 16.2 meq O2/kg under accelerated aging at 55 °C for 27 days, equivalent to 18 months at room temperature. Oxidised fatty acids increased in fortified spaghetti from 4.8 to 13.8 cc of fat under light exposure over 12 months and from 4.8 to 7.8 g/100 g of fat at 55 °C in 27 days. The high sensitivity of spectrophotometric and chromatographic analytical methods permitted the evaluation of primary and secondary oxidative derivatives in small amounts of fat (Verardo et al., 2009).