2. Materials and methods
2.1. Materials
Fresh and mature coconuts (10–12 months old) were procured
from the local market. Commercial coconut oil sample is procured
from the market (Departmental Store, Mysore). Enzyme aspartic
protease [EC 3.4.23] (Activity: 2500 tyrosine units/g) of commercial
grade was procured from Kaypeeyes Biotech Private Ltd., Mysore,
India. All chemicals of analytical grade were procured from
Merck Chemicals, Mumbai, India. For GC analysis, hexane (HPLC
grade) was procured from Ranbaxy Fine Chemicals Ltd, Mumbai,