Tannase (EC 3.1.1.20) is a hydrolytic enzyme involved in the biodegradation of tannins. This enzyme catalyzes the hydrolysis of the ester and depside bonds present in hydrolysable tannins, releasing gallic acid and glucose [1]. Tannase also has many applications in food, bev- erage, pharmaceutical and chemical industries, and even in bioremediation [2,3]. Industrial bioconversion of tan- nic acid is generally accomplished by tannase for the production of gallic acid, which is mostly used in the pharmaceutical industry to produce the anti-bacterial drug Trimethoprim [4]. Gallic acid is also an important substrate for the synthesis of propyl gallate, an antioxi- dant used in the food industry [1].
Microorganisms can continuously produce tannase in large quantities, resulting in an increased yield with the establishment of optimized fermentation methods. Tan- nase is produced in the presence of tannic acid by several filamentous fungi, mainly Aspergillus and Penicillium species [4]. Industrial production of microbial tannase is obtained by submerged culture because this simplifies sterilization and process control [5]. Studies evaluating the choosing strains and enzymes with desirable charac- cultivation of tannase-producing strains are important for
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Tannase (EC 3.1.1.20) is a hydrolytic enzyme involved in the biodegradation of tannins. This enzyme catalyzes the hydrolysis of the ester and depside bonds present in hydrolysable tannins, releasing gallic acid and glucose [1]. Tannase also has many applications in food, bev- erage, pharmaceutical and chemical industries, and even in bioremediation [2,3]. Industrial bioconversion of tan- nic acid is generally accomplished by tannase for the production of gallic acid, which is mostly used in the pharmaceutical industry to produce the anti-bacterial drug Trimethoprim [4]. Gallic acid is also an important substrate for the synthesis of propyl gallate, an antioxi- dant used in the food industry [1]. Microorganisms can continuously produce tannase in large quantities, resulting in an increased yield with the establishment of optimized fermentation methods. Tan- nase is produced in the presence of tannic acid by several filamentous fungi, mainly Aspergillus and Penicillium species [4]. Industrial production of microbial tannase is obtained by submerged culture because this simplifies sterilization and process control [5]. Studies evaluating the choosing strains and enzymes with desirable charac- cultivation of tannase-producing strains are important for 144
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