To assay the chemical composition of wood wastes, the organic
solvent soluble and hot-water soluble compounds were analyzed
using the United States National Renewable Energy Laboratory
(NREL) procedure with the modification of using acetone instead
of ethanol–benzene as the solvent (NREL, 2005). Acetone and
hot-water extracted C&D wood waste were used for Klason lignin
determination and carbohydrate compositional analysis. The
Klason lignin content was analyzed by the NREL procedure but
the carbohydrate composition was analyzed by acid hydrolysis followed
by 1H-NMR spectroscopy (NREL, 2008; Shin and Cho, 2008).
Acidic filtrates were analyzed by Bruker Avance NMR Spectrometer
(600 MHz for proton). NMR data were processed by Win NMR software
to resolve and integrate the NMR peaks. Anomeric hydrogen
peak regions (4.4–5.4 ppm) in NMR spectra were integrated for
carbohydrate composition. Due to the hydrogen exchange with hydroxyl
groups in monosaccharides, HDO was formed and could be
interfered in anomeric hydrogen peak assignment. To avoid this
problem, we made sample as closely to NMR analysis point and
we could adjust that problem with the changes in acidity. For accurate
peak assignment, we repeated with standard monosaccharide
with similar acidity condition.
To assay the chemical composition of wood wastes, the organicsolvent soluble and hot-water soluble compounds were analyzedusing the United States National Renewable Energy Laboratory(NREL) procedure with the modification of using acetone insteadof ethanol–benzene as the solvent (NREL, 2005). Acetone andhot-water extracted C&D wood waste were used for Klason lignindetermination and carbohydrate compositional analysis. TheKlason lignin content was analyzed by the NREL procedure butthe carbohydrate composition was analyzed by acid hydrolysis followedby 1H-NMR spectroscopy (NREL, 2008; Shin and Cho, 2008).Acidic filtrates were analyzed by Bruker Avance NMR Spectrometer(600 MHz for proton). NMR data were processed by Win NMR softwareto resolve and integrate the NMR peaks. Anomeric hydrogenpeak regions (4.4–5.4 ppm) in NMR spectra were integrated forcarbohydrate composition. Due to the hydrogen exchange with hydroxylgroups in monosaccharides, HDO was formed and could beinterfered in anomeric hydrogen peak assignment. To avoid thisproblem, we made sample as closely to NMR analysis point andwe could adjust that problem with the changes in acidity. For accuratepeak assignment, we repeated with standard monosaccharidewith similar acidity condition.
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