Fig. 6 demonstrates the CL emission spectra for luminol–H2O2
CL system in the presence of CuO nanosheets and cloxacillin
sodium. As can be seen from Fig. 6, the maximum emission for
the luminol–H2O2 and luminol–H2O2–CuO nanosheets is located
at 425 nm and only the intensity of CL spectrum is increased for
luminol–H2O2–CuO nanosheets CL system. Based on these results,
it can be proposed that the main emitter of the luminol–H2O2 CL
system in the presence of CuO nanosheets is still the 3-AMP*. So,
the enhancement effect of CuO nanosheets can be related to the
accelerated production of 3-AMP and 3-AMP*, leading to the higher
CL intensities.
In addition, from Fig. 6, it can be clearly seen that the maximum
emission wavelength of the luminol–H2O2–CuO nanosheets in the
presence of cloxacillin sodium is also located at 425 nm, with
increased CL emission at kmax = 425 nm. This approves that the
addition of cloxacillin sodium into the luminol–H2O2 system does
not generate a new luminophor and the emitter is again 3-AMP*.
The increase of CL spectrum in the presence of cloxacillin sodium
can be attributed to the increased production of 3-AMP* ions. It
can be inferred that, H2O2 can oxidize cloxacillin sodium by the
catalysis of CuO nanosheets, leading to the production of excited
state oxidized cloxacillin sodium (cloxacillin sodium*). When the
cloxacillin sodium* returns to the state of ground, it can transfer
energy to the 3-AMP, this leads to the generation of more
3-AMP* ions, the more 3-AMP* ions return to the state of ground
with increased CL emission. The reaction mechanism of luminol–
H2O2 in the presence of cloxacillin sodium is proposed in Eqs.
Fig. 6 demonstrates the CL emission spectra for luminol–H2O2CL system in the presence of CuO nanosheets and cloxacillinsodium. As can be seen from Fig. 6, the maximum emission forthe luminol–H2O2 and luminol–H2O2–CuO nanosheets is locatedat 425 nm and only the intensity of CL spectrum is increased forluminol–H2O2–CuO nanosheets CL system. Based on these results,it can be proposed that the main emitter of the luminol–H2O2 CLsystem in the presence of CuO nanosheets is still the 3-AMP*. So,the enhancement effect of CuO nanosheets can be related to theaccelerated production of 3-AMP and 3-AMP*, leading to the higherCL intensities.In addition, from Fig. 6, it can be clearly seen that the maximumemission wavelength of the luminol–H2O2–CuO nanosheets in thepresence of cloxacillin sodium is also located at 425 nm, withincreased CL emission at kmax = 425 nm. This approves that theaddition of cloxacillin sodium into the luminol–H2O2 system doesnot generate a new luminophor and the emitter is again 3-AMP*.The increase of CL spectrum in the presence of cloxacillin sodiumcan be attributed to the increased production of 3-AMP* ions. Itcan be inferred that, H2O2 can oxidize cloxacillin sodium by thecatalysis of CuO nanosheets, leading to the production of excitedstate oxidized cloxacillin sodium (cloxacillin sodium*). When thecloxacillin sodium* returns to the state of ground, it can transferenergy to the 3-AMP, this leads to the generation of more3-AMP* ions, the more 3-AMP* ions return to the state of groundwith increased CL emission. The reaction mechanism of luminol–H2O2 in the presence of cloxacillin sodium is proposed in Eqs.
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