As shown in Fig. 4a, the biomass of T. spathulata increased when ammonium sulfate was added. It
reached the maximum value of 10.15 g/L at 0.5% ammonium sulfate. At this concentration, the maximum
lipid yield was also obtained (4.13 g/L). Further rise in ammonium sulfate concentration beyond 0.5%
resulted in a drop of biomass, lipid yield and lipid content. In Fig. 4b, biomass of K. ohmeri increased
gradually with the increase of ammonium sulfate concentration and reached the maximum biomass of
10.45 g/L at 0.5% ammonium sulfate. Lipid yield showed a sharp increase when ammonium sulfate
concentration increased from 0.25% to 0.5%, and reached the maximum lipid yield of 3.17 g/L at 0.5%.
From Fig. 4, it can be seen that at lower concentration of nitrogen source, the lipid content was higher.
This result indicated that the exhaustion of ammonium-N apparently greatly promoted lipid accumulation.
The similar results were also observed in lipid content of Rhodosporidium toruloides Y4, Rhodotorula
glutinis and Trichosporon fermentans [11].
As shown in Fig. 4a, the biomass of T. spathulata increased when ammonium sulfate was added. Itreached the maximum value of 10.15 g/L at 0.5% ammonium sulfate. At this concentration, the maximumlipid yield was also obtained (4.13 g/L). Further rise in ammonium sulfate concentration beyond 0.5%resulted in a drop of biomass, lipid yield and lipid content. In Fig. 4b, biomass of K. ohmeri increasedgradually with the increase of ammonium sulfate concentration and reached the maximum biomass of10.45 g/L at 0.5% ammonium sulfate. Lipid yield showed a sharp increase when ammonium sulfateconcentration increased from 0.25% to 0.5%, and reached the maximum lipid yield of 3.17 g/L at 0.5%.From Fig. 4, it can be seen that at lower concentration of nitrogen source, the lipid content was higher.This result indicated that the exhaustion of ammonium-N apparently greatly promoted lipid accumulation.The similar results were also observed in lipid content of Rhodosporidium toruloides Y4, Rhodotorulaglutinis and Trichosporon fermentans [11].
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