results of swarming assays and the predicted ternary
structures of CheB1 and CheB3 of L. interrogans strain
Lai suggested that the absence of an N-terminal regulatory
domain may be one of the reasons for the failure of CheB2
to complement an E. coli cheB mutant. Furthermore,
CheB2 links solely to CheR1 and CheR3 in the interaction
network of leptospires. Taken together, these results indicated
that CheB2 may not function alone, and under
certain physiological conditions, it may require CheB3 and
CheR1 to function. The existence of multiple copies of chemotaxis
gene homologs suggested that L. interrogans strain
Lai might have a more complex chemosensory pathway.