This study was carried
out at theexperimental facility of Shahid Rajaee Fish Farm
located in northern part of Iran. R.kutum larvae were breed
at the reproduction facility of the fish farm and adapted to
the experimental conditions a few days beforethestart of
the experiment. Afterward, the fish with an average weight
of 300 mgwere divided randomly into twelve 500L tanks
with an initial stocking density of 500 individual per tank.
The experiment lastedfor 8 weeks. Prebiotic used in this
study, Immunogen, is composed ofmannanoligosaccharide
(18%) and -glucans (1-3, 1-6) (30%).
(Provided by Soroush Radian Co., Tehran, Iran)