cleaning and reactivation of the electrode surface achieved by
applying an appropriate pulse potential waveform. The technique,
known as pulsed amperometry, was successfully applied to
address surface-related challenges associated with alcohols,
carbohydrates, amino acids, and organo-sulfur compounds
detection [37–40]. By this way the precision of the determinations
is greatly improved. The efficiency of the suggested approach was
recently demonstrated performing organophosphorus pesticides
(OPs) quantification by applying an organophosphorus hydrolase-
based electrochemical sensor [41]. The oxidation current of
p-nitrophenol released upon organophosphorus hydrolase-cata-
lyzed hydrolysis of paraoxon, parathion and methyl parathion,
which is directly proportional to the OPs concentration, was
recorded as analytical signal. RSD of 0.41% at 40 mmol L1
p-nitrophenol determination was achieved after 10 determinations,
instead of 43.93% without the pulsed cleaning application
cleaning and reactivation of the electrode surface achieved by
applying an appropriate pulse potential waveform. The technique,
known as pulsed amperometry, was successfully applied to
address surface-related challenges associated with alcohols,
carbohydrates, amino acids, and organo-sulfur compounds
detection [37–40]. By this way the precision of the determinations
is greatly improved. The efficiency of the suggested approach was
recently demonstrated performing organophosphorus pesticides
(OPs) quantification by applying an organophosphorus hydrolase-
based electrochemical sensor [41]. The oxidation current of
p-nitrophenol released upon organophosphorus hydrolase-cata-
lyzed hydrolysis of paraoxon, parathion and methyl parathion,
which is directly proportional to the OPs concentration, was
recorded as analytical signal. RSD of 0.41% at 40 mmol L1
p-nitrophenol determination was achieved after 10 determinations,
instead of 43.93% without the pulsed cleaning application
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