Culture conditions and statistical analysis Cultures were incubated at 25 ± 2 ºC under a light intensity of 30 µM m-2 s-1 obtained with white fluorescent lamps and a 16 h photoperiod. The experimental design was a randomized complete block in a two-way factorial arrangement (2 x 2) with four replicates of 40 explants per treatment. The data was submitted to a normality analysis by the Lilliefors´s method, followed by the analysis of variance (ANOVA), followed by the Duncan’s multiple range test with the level of significance