A strain of A.niger 20 was used in this study. This strain was previously identified as an efficient bioremediation fungal strain [19]. The fungal strain was preserved on potato agar slants medium containing (g/L) peeled potatoes (200), glucose (20), and yeast extract (0.1) in a refrigerator at 4 °C and was sub-cultured every 4 months. A sucrose medium was used for inoculum preparation and biomass culture. The composition of this culture medium was as follows: 10 g/L of sucrose, 0.5 g/L of H3PO4, 0.2 g/L of MgSO4 ·7H2O, and 0.1 g/L of NaCl [8], pH in nature.