A new method based on the cholesterol level was developed to detect the presence of animal fats in virgin
coconut oil (VCO). In this study, the sterols in VCO and animal fats was separated using conventional onedimensional
gas chromatography (1D GC) and comprehensive two-dimensional gas chromatography
(GC GC). Compared with 1D GC, the GC GC system could obtain a complete baseline separation of
the sterol trimethylsilyl ethers derived from cholesterol and cholestanol, so that the cholesterol content
in pure VCO and false VCO adulterated with animal fats could be accurately determined. Cholesterol, a
main sterol found in animal fats, represented less than 5 mg/kg of VCO. The study demonstrated that
the determination of the cholesterol level in VCO could be used for reliable detection of the presence
of lard, chicken fat, mutton tallow, beef tallow, or their mixture in VCO at a level as little as 0.25%.
A new method based on the cholesterol level was developed to detect the presence of animal fats in virgincoconut oil (VCO). In this study, the sterols in VCO and animal fats was separated using conventional onedimensionalgas chromatography (1D GC) and comprehensive two-dimensional gas chromatography(GC GC). Compared with 1D GC, the GC GC system could obtain a complete baseline separation ofthe sterol trimethylsilyl ethers derived from cholesterol and cholestanol, so that the cholesterol contentin pure VCO and false VCO adulterated with animal fats could be accurately determined. Cholesterol, amain sterol found in animal fats, represented less than 5 mg/kg of VCO. The study demonstrated thatthe determination of the cholesterol level in VCO could be used for reliable detection of the presenceof lard, chicken fat, mutton tallow, beef tallow, or their mixture in VCO at a level as little as 0.25%.
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