Macroinvertebrate Index Sampling
To analyze water quality using benthic macroinvertebrate index we collected three samples at each in stream site using a 30‐centimeter square surber sampler. After each sample the collected material was transferred to a large pan by turning the net inside out and then using a spray bottle to rinse the net clean and capture all invertebrates. Forceps were used to remove organisms from the sand and debris and then to deposit them into a sample bottle filled with river water. The collected macroinvertebrates were later identified in the laboratory using hand‐lenses and microscopes and then counted to perform an EPT index (Kitchin 1995; Watson‐Fergeson 2006).