The field intensity of 0.5 mT did not exerted any detectable effect on blastocysts development when compared to CTR (p > 0.05) independently of the protocol used. On the contrary, fields of higher intensities (0.75 and 1 mT), were able to slow the embryo cleavage: in fact, in both protocols approximately 25% of embryos reached the morula stage and the remaining blastocysts isolated displayed a mean number of blastomers significantly lower (p < 0.01) than those recorded in CTR blastocysts.
3.3. Oviductal morphology
ELF-EMF in vivo exposed oviducts at all intensities considered excised 12 h and 6 days post-fertilization, after PA or PB treatment, were morphologically analysed, together with their contra lateral controls, in order to verify if ELF-EMF could cause any damage to this female genital tract.
All samples had a typical morphology and only slight petechiae were visible at the tubal site exposed to ELF-EMF.
The examined histological sections of both treated and CTR oviductal segments (UTJ, I, AIJ, and A) showed no inflammatory reaction (Fig. 5), as confirmed by HE and HE-AB staining.
The field intensity of 0.5 mT did not exerted any detectable effect on blastocysts development when compared to CTR (p > 0.05) independently of the protocol used. On the contrary, fields of higher intensities (0.75 and 1 mT), were able to slow the embryo cleavage: in fact, in both protocols approximately 25% of embryos reached the morula stage and the remaining blastocysts isolated displayed a mean number of blastomers significantly lower (p < 0.01) than those recorded in CTR blastocysts.
3.3. Oviductal morphology
ELF-EMF in vivo exposed oviducts at all intensities considered excised 12 h and 6 days post-fertilization, after PA or PB treatment, were morphologically analysed, together with their contra lateral controls, in order to verify if ELF-EMF could cause any damage to this female genital tract.
All samples had a typical morphology and only slight petechiae were visible at the tubal site exposed to ELF-EMF.
The examined histological sections of both treated and CTR oviductal segments (UTJ, I, AIJ, and A) showed no inflammatory reaction (Fig. 5), as confirmed by HE and HE-AB staining.
การแปล กรุณารอสักครู่..