Background: A rapid and sensitive ultraviolet-visible (UV-VIS) spectroscopic method was developed for the estimation
of pyrimidine derivative 6-Bromo-3-(6-(2,6-dichlorophenyl)-2-(morpolinomethylamino) pyrimidine4-yl) -2H-chromen-2-
one (BT10M) in bulk form.
Results: Pyrimidine derivative was monitored at 275 nm with UV detection, and there is no interference of diluents at
275 nm. The method was found to be linear in the range of 50 to 150 μg/ml. The accuracy and precision were
determined and validated statistically. The method was validated as a guideline.
Conclusions: The results showed that the proposed method is suitable for the accurate, precise, and rapid
determination of pyrimidine derivative.
Keywords: Pyrimidine; Derivative; UV-VIS spectroscopy; Validation