Thirty-twolambsweredividedin4groupswith2replicateseach(8batchesintotal)
accordingtotheirbodyweights.Thelambswerefedatotalmixedration(TMR)formulated
eitherwithpalmoil(CTRL, 2replicates,4animalsperreplicate;34gpalmoilkg1 TMR)or
flaxseed(FS, 2replicates,4animalsperreplicate;85gflaxseedkg1 TMR).Fourmore
batcheswerefedthesameTMRsbutenrichedwithquercetin(QCT, 2replicates,4animals
perreplicate,34gpalmoilplus2gquercetinkg1 TMR; FS–QCT, 2 replicates,4animalsper
replicate,85gflaxseedplus2gquercetinkg1 TMR).Threeweeksafterstartingwiththe
experimentaldiets,theanimalswereimmunizedbyasubcutaneousinjectionofovalbumin,
andbloodsampleswerecollectedatdays0,4,9,14and17post-immunizationtomeasure
antibodiesagainstovalbumin.Afterwards,allofthelambsweresubjectedtoa4-h
transportation-stressperiodtostudytheevolutionofhaematologicalandbiochemical
parametersduringroadtransportand,finally,slaughtered.Meanova-specificIgGtitres
weresignificantlylowerinthelambsfedFSondays14and17whencomparedtoCTRL
group(FSDAY, P¼0.033).Additionally,FSreducedwhitebloodcellscountsandtissue
damage(creatinephosphokinase, Po0.05)duringroadtransport.Consequently,flaxseed
showedsomeimmunologicalpropertiesandprotectionagainsttissuedamageduringroad
transport.Regardingruminalacidosis,bothquercetinandflaxseedseemedtobeadequateto
reducethelevelofparakeratosis.
Thirty-twolambsweredividedin4groupswith2replicateseach(8batchesintotal)accordingtotheirbodyweights Thelambswerefedatotalmixedration (TMR) สูตรeitherwithpalmoil (CTRL, 2replicates, 4animalsperreplicate; 34gpalmoilkg 1 TMR) หรือเมล็ดแฟลกซ์ (FS, 2replicates, 4animalsperreplicate; 85gflaxseedkg 1 TMR) FourmorebatcheswerefedthesameTMRsbutenrichedwithquercetin (QCT, 2replicates, 4animalsperreplicate, 34gpalmoilplus2gquercetinkg 1 TMR FS-QCT, 2 สามารถจำลอง 4animalsperจำลอง 85gflaxseedplus2gquercetinkg 1 TMR) Threeweeksafterstartingwiththeexperimentaldiets, theanimalswereimmunizedbyasubcutaneousinjectionofovalbuminandbloodsampleswerecollectedatdays0, 4, 9, 14and17post-immunizationtomeasureantibodiesagainstovalbumin ภายหลัง allofthelambsweresubjectedtoa4-hขนส่ง-stressperiodtostudytheevolutionofhaematologicalandbiochemicalparametersduringroadtransportand สุด ฆ่า Meanova-specificIgGtitresweresignificantlylowerinthelambsfedFSondays14and17whencomparedtoCTRLกลุ่ม (FS วัน P¼0.033) นอกจากนี้ FSreducedwhitebloodcellscountsandtissueความเสียหาย (creatinephosphokinase, Po0.05) duringroadtransport ดังนั้น เมล็ดแฟลกซ์showedsomeimmunologicalpropertiesandprotectionagainsttissuedamageduringroadขนส่ง Regardingruminalacidosis, bothquercetinandflaxseedseemedtobeadequatetoreducethelevelofparakeratosis
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2replicates, 4animalsperreplicate; 34gpalmoilkg 1 TMR) หรือflaxseed (FS, 2replicates, 4animalsperreplicate; 85gflaxseedkg 1 TMR) .Fourmore? batcheswerefedthesameTMRsbutenrichedwithquercetin (QCT, 2replicates, 4animals perreplicate, 34gpalmoilplus2gquercetinkg 1 TMR; FS-QCT 2 ซ้ำ, 4animalsper ซ้ำ 85gflaxseedplus2gquercetinkg 1 P¼0.033) .Additionally, FSreducedwhitebloodcellscountsandtissue ความเสียหาย (creatinephosphokinase,
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thirty-twolambsweredividedin4groupswith2replicateseach ( 8batchesintotal )
accordingtotheirbodyweights . thelambswerefedatotalmixedration ( TMR ) สูตร
eitherwithpalmoil ( Ctrl 2replicates 4animalsperreplicate ; 34gpalmoilkg 1 , TMR ) หรือ
flaxseed ( FS , 2replicates 4animalsperreplicate ; 85gflaxseedkg , 1 แบบ ) fourmore
batcheswerefedthesametmrsbutenrichedwithquercetin ( qct 2replicates perreplicate 4animals
, , ,34gpalmoilplus2gquercetinkg 1 โค ; FS – qct 2 ซ้ำ 4animalsper
, มาก 85gflaxseedplus2gquercetinkg 1 แบบ ) threeweeksafterstartingwiththe experimentaldiets theanimalswereimmunizedbyasubcutaneousinjectionofovalbumin
, , andbloodsampleswerecollectedatdays0,4,9,14and17post-immunizationtomeasure allofthelambsweresubjectedtoa4-h
antibodiesagainstovalbumin หลังจากนั้นการขนส่ง stressperiodtostudytheevolutionofhaematologicalandbiochemical
parametersduringroadtransportand ในที่สุดฆ่า meanova specificiggtitres
weresignificantlylowerinthelambsfedfsondays14and17whencomparedtoctrl กลุ่ม ( FS วัน , p ¼ 0.033 ) นอกจากนี้ fsreducedwhitebloodcellscountsandtissue
ความเสียหาย ( creatinephosphokinase po0.05 , flaxseed
) duringroadtransport จากนั้นshowedsomeimmunologicalpropertiesandprotectionagainsttissuedamageduringroad
การขนส่ง regardingruminalacidosis bothquercetinandflaxseedseemedtobeadequateto
, reducethelevelofparakeratosis .
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