Temperature design
To reduce stress to the animals, temperatures were
changed gradually (2ć per 3-4 days) and animals were
monitored to ensure that they fed normally for at least
two successive feedings before temperature increased
or decreased further. During the experimental period,
the water temperature was continuously monitored
by a controlling device and adjusted with ice or warm
water as necessary to remain at the target (4, 6, 8, 10,