3.3. Performance of the method for three different growth mediain comparisonA 7-band pattern of TCY (0.5–10 ng/band) was applied on theHPTLC plate. Each pattern (not developed) was subjected to the B.s. bioassay using various growth media, i.e. HB, LB and MLB with0.7 ≤ OD600 nm≤ 0.9. After visualization with the MTT solution, thedensitometric measurement was performed and images were cap-tured under white light illumination in the reflectance mode. Withthis pretest, various parameters were studied in the following. Thebest growth medium was used for a real sample analysis later on.