The skin was damaged in the wound groups, and this resulted in decreased absorptivity. In wound
animal groups, we demonstrated that TDD of BB oil at three different concentrations (100%, 50% and
20%) did not cause any significant effect on liver as observed with the levels of ALT, AST, ALP and
TBili. All concentrations of BB oil showed a decrease in serum index compared to the normal
animal groups, suggesting that wound affected skin absorptivity. These results were confirmed with
histopathology analysis of liver samples, where no significant lesions or acute hepatotoxicity
were observed.
In our recent study, 100% w/v BB oil applied to intact and damaged skin for 24 h showed no
significant allergy or acute toxicity [31]. This study was carried out to further evaluate the short term
adverse reactions of human consumption of BB oil. The levels of serum index and liver histopathology
confirmed the safety of high BB oil concentrations. In addition, our data confirmed that no significant
positive correlation exists between BB oil absorption and concentration. However, further
investigations are required to clarify this issue.
Since essential oils have good solubility in vegetable oil, the vehicle used herein was olive oil
instead of DMSO or other organic solvents [32–34]. It tends to completely dissolve and diffuse the BB
oil. Olive oil, which is often employed in cosmetic dermatology, has been reported to have a