2.7. Characterization of fitness costs
Seeds of resistant (BrI-637) and sensitive (BrI-638) accessions were germinated in a dark cold room (16◦C) to break seeds’ dormancy. Six uniform seedlings of each accession were transplanted into pots (7 × 7 × 6 cm), which were moved to a net house (16◦C night/28◦C day). After 71 days, the above ground biomass was collected, oven-dried (80◦C) and weighed. Plant shoot dry weights were calculated.