Standard stock solutions of BZL and PHY (1mg
mL1) were prepared separately by dissolving 10 mg
of respective materials in the mixture of acetonitrile
and phosphate buffer (80:20 % v/v) and the volume
was made up to 10 mL and it was stored under
refrigerator condition. Further to study the linearity
response of the BZL a serial dilutions from 0.1 to 1.5
μg mL-1were prepared from the standard stock
solution of BZL. A graph was plotted between
concentration of drugs and peak area response