Soares and Gouveia [22] performed the alkaline extraction ofcane bagasse in a water bath for 30 min using 0.5 and 1.0 wt%NaOH at 10 wt% TS, resulting in 51.8 and 76.9% delignification,respectively. Oliveira et al. [2] used 1.5 wt% NaOH at 10 wt% TSand obtained a delignification of 66.5% after 60 min at 100◦C.Under similar alkaline delignification conditions, Khuong et al. [31]applied 1.0 wt% NaOH at 5 wt% TS and obtained an alkali-washedsubstrate with 52.8% glucans, 9.4% xylans, and 7.6% total lignin fora total delignification level of 63.8%.The mass recovery after pretreatment agreed well with thedelignification data, with the highest level of the former corre-sponding to the smallest level of the latter. High glucan recoverieswere obtained throughout the entire experimental design. In manysituations, glucan recoveries higher than 100% were obtained andthis was attributed to uncertainties in the analytical proceduresinvolved in this study.