To illustrate the application to environmental samples, the enzymatic inorganic phosphate method with green chemistry described herein has also been employed to analyze water samples. A set of inorganic phosphate standards with a range from 5 to 50 mg Phosphate per L, was used for establishing the standard curve with 100 μL sample size in a 1.0 mL assay final volume. Each standard was analyzed 6 times and the mean absorbance at 360 nm (corrected for background absorbance 360 nm) was plotted versus the nominal inorganic phosphate concentration. These data were fitted with linear regression and the equation of the fitted line was: