Acid invertase was extracted from mango nectars using 4% (w/v)
NaCl solution as reported by Hasegawa and Smolensky (1970). Invertase
assay was performed using sucrose as a substrate as described earlier
(Bhatti, Asgher, Abbas, Nawaz, & Sheikh, 2006) with some
modifications. An appropriate amount (0.5 mL) of the enzyme solution
was mixed with 5 mL of pH 4.0 sodium phosphate–citric acid buffer
(contained 2% sucrose, w/v) and shaken in a water bath at 55 °C for
6 h. The reaction was quenched by placing tubes in boiling water for
10 min, and immediately cooling in an ice bath. The released glucose
Acid invertase was extracted from mango nectars using 4% (w/v)NaCl solution as reported by Hasegawa and Smolensky (1970). Invertaseassay was performed using sucrose as a substrate as described earlier(Bhatti, Asgher, Abbas, Nawaz, & Sheikh, 2006) with somemodifications. An appropriate amount (0.5 mL) of the enzyme solutionwas mixed with 5 mL of pH 4.0 sodium phosphate–citric acid buffer(contained 2% sucrose, w/v) and shaken in a water bath at 55 °C for6 h. The reaction was quenched by placing tubes in boiling water for10 min, and immediately cooling in an ice bath. The released glucose
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