Insights about the distribution of the microbial community prove to be the major goal of understanding microbial
ecology which remains to be fully deciphered. Hot springs being hub for the thermophilic microbiota attract the
attention of the microbiologists. Deulajhari hot spring cluster is located in the Angul district of Odisha. Covered
within a wooded area, Deulajhari hot spring is also fed by the plant litter resulting in a relatively high amount
of total organic content (TOC). For the first time, Illumina sequencing based biodiversity analysis of microbial
composition is studied through amplicon metagenome sequencing of 16s rRNA targeting V3 ‐V4 region using
metagenomic DNA from the hot spring sediment. Over 2 8 phyla were detected through the amplico n
metagenome sequencing of which the most dominating phyla at the existing physiochemical parameters like;
temperature 69 ° C, pH 8.09, electro conductivity 0.025 dSm− 1
and to tal or ganiccarbon 0.3 56 % , were
Proteobacteria ( 88.12% ) ,Bacteriodetes ( 10.76 % ), Firmicutes ( 0.35 % ), Spirochetes ( 0.18 %) and chloroflexi
(0.11% ). Approximately 713 species were observed at the above physiochemical parameters. The analysis of
the metagenome provides the quantitative insights into microbial populations based on the sequence data in
Deulajhari hot spring. Metagenome sequence is deposited to SRA database which is available at NCBI with accession no. SRX1459736.